Glucose-6phosphate dehydrogenase {G6PD} Test.

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Glucose-6phosphate dehydrogenase {G6PD} Test.

Glucose-6phosphate dehydrogenase {G6PD} Test.

Definition :-

– G6PD is an enzyme normally present in RBCs as part of the pentose phosphate pathway that metabolizes glucose and antioxidant to protect cells from damage by free radicals.

– G6PD deficiency is sex-linked genetic disorder found mostly in males [like hemophilia] that results in haemolysis of erythrocytes producing anemia after the recept of certain drugs or fava beans.

– By electrophoretic technique there are 3 clinical important types of G6PD deficiencies :-

1- G6PD   A: – common in blacks.

2- G6PD Mahidol:  common in south east Asians.

3- G6PD  Mediterranean :-  Characterized by sever and sometimes fetal hemolysis following eating fava beans.

 Clinical significance :-

– Decreased in :- Favism , conginital G6PD deficiency

Specimen and collection :-

Don’t use oxalate or fluoride as anticoagulants.

Methododology :-

Hemolysated of washed RBCs.

Guiding Reference Value :

> 30% of enzyme amount.

Limitation :-

– G6PD is very important to RBCs due to generating of NADPH, which is necessary to prevent methemoglobin accumulation.

– Many drugs cause G6PD deficiency such as: – antimalarials , naldixic acid , nitrofurantoin , phenacetin , sulfones., large dose of vitamin C and some sulfonamides.

Glucose-6phosphate dehydrogenase {G6PD} Test.

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